Insights into the chloroplast genome diversity of the genus Isatis in China.

ABSTRACT:

Background

The genus Isatis contains medicinally important but taxonomically controversial species in China. Reliable genomic resources are urgently needed for accurate species identification and phylogenetic clarification.

Results

We assembled and characterized the complete chloroplast genomes of seven Isatis species. The genomes (153,260-153,872 bp) exhibited the typical quadripartite structure. Sequence variation was heterogeneous: the small single-copy (SSC) region was the most polymorphic (12.3 single-nucleotide polymorphisms (SNPs)/kb), followed by the large single-copy (LSC, 8.9 SNPs/kb) and inverted repeat (IR, 1.7 SNPs/kb) regions. Noncoding sequences showed 3.2-fold greater polymorphism than coding sequences. The rpl32-trnL intergenic spacer was identified as a pro

SUBMITTER: Wei M 

PROVIDER: S-EPMC12914916 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature