A Modular Vaccine Platform Against SARS-CoV-2 Based on Self-Assembled Protein Nanoparticles.

ABSTRACT: The COVID-19 pandemic has underscored the urgent need for deployable vaccine platforms capable of rapidly responding to emerging and re-emerging variants of human coronaviruses. BP26, an outer membrane protein of the zoonotic bacterium Brucella, self-assembles into a highly ordered, barrel-like nanoparticle that can serve as a vaccine platform, demonstrating immunogenicity against the influenza virus and cancer when combined with specific antigens. Here, we expanded its versatility by incorporating the SpyTag/SpyCatcher pair, enabling modular and site-specific antigen conjugation via simple mixing. SpyCatcher was genetically fused to BP26, and SpyTag to the receptor binding domain (RBD) of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein, generating RBD-displa

SUBMITTER: Lee S 

PROVIDER: S-EPMC12915080 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature