An Automated Organotypic SCN Culture System Revealing Novel Insights into VIP Regulation of Circadian Rhythm.

ABSTRACT: In mammals, the suprachiasmatic nucleus (SCN) functions as the primary driver of circadian rhythm. Although individual SCN neurons exhibit cell-autonomous oscillations, intercellular communication-mediated by neural peptides such as vasoactive intestinal polypeptide (VIP)-synchronizes their rhythms. However, the precise molecular mechanism by which VIP facilitates SCN synchronization remains elusive. Here, we introduce an automated ex vivo culture system, termed 'brain-slice-in-a-chamber' (BaSIC), tailored for SCN slices, as well as dissociated cells culture. This apparatus automates medium exchange and ensures optimal control over temperature and humidity, ensuring a stable internal environment conducive to tissue culture. Furthermore, BaSIC enables real-time observation of tissue respons

SUBMITTER: Han K 

PROVIDER: S-EPMC12915189 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature