Contrasting effects of glutamate and branched-chain amino acid metabolism on acid tolerance in a <i>Castellaniella</i> isolate from acidic groundwater.

ABSTRACT: Groundwater acidification co-occurring with nitrate pollution is a common, global environmental health hazard. Denitrifying bacteria have been leveraged for the in situ removal of nitrate in groundwater. However, co-existing stressors-such as low pH-reduce the efficacy of biological removal processes. Castellaniella sp. str. MT123 is a complete denitrifier that was isolated from acidic, nitrate-contaminated groundwater. The strain grows robustly by nitrate respiration at pH < 6.0, completely reducing nitrate to dinitrogen gas. Genomic analyses of MT123 revealed few previously characterized acid tolerance genes. Thus, we utilized a combination of proteomics, metabolomics, and competitive mutant fitness to characterize the genetic mechanisms of MT123 acclimation to growth under

SUBMITTER: Goff JL 

PROVIDER: S-EPMC12915299 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature