Structural and functional insights into Uly1040, an ulvan lyase from polysaccharide lyase family 40.

ABSTRACT: Ulvan is a major polysaccharide in marine green algae. Its oligosaccharide degradation products possess diverse bioactivities and hold considerable potential for various applications. Ulvan lyases, the key enzymes responsible for cleaving ulvan glycosidic bonds, generate bioactive oligosaccharides and play an essential role in ulvan degradation. However, studies on ulvan lyases remain limited, particularly for the poorly characterized polysaccharide lyase (PL) 40 family. Here, we identified Uly1040, a novel PL40 ulvan lyase, from the marine bacterium Alteromonas macleodii isolated from the intestine of an Aplysia sea slug. Uly1040 displays a unique two-domain architecture not previously reported in ulvan lyases. Mechanistically, Uly1040 employs a distinct His/Tyr catalytic st

SUBMITTER: Wang H-Q 

PROVIDER: S-EPMC12915356 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature