The Molecular Cochaperone NbSGT1 May Function as an Endogenous Suppressor of RNA Silencing That Is Recruited by a Potyvirus in Infection of Plants.

ABSTRACT: Recent evidence indicates that plant cells contain specific endogenous suppressors of RNA silencing (ESRs) that plant viruses can exploit to counteract host defences. However, the underlying mechanisms are not yet fully understood. The helper component-proteinase (HC-Pro) of potyviruses is known to suppress RNA silencing and facilitate viral infection. Here, we used affinity purification followed by mass spectrometry to identify potential host proteins that interact with HC-Pro during telosma mosaic virus (TelMV, genus Potyvirus) infection in plants. We found that the molecular co-chaperone SGT1 (suppressor of the G2 allele of Skp1) interacts with HC-Pro via its SGS domain. Virus-induced gene silencing and RNAi-mediated knockdown of NbSGT1 resulted in decreased viral accumulation in Nicoti

SUBMITTER: Shi W 

PROVIDER: S-EPMC12916245 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature