Single-cell multimodal analysis reveals the dynamic immunopathogenesis of HBV-ACLF progression.

ABSTRACT:

Background

Acute-on-chronic liver failure (ACLF) is a life-threatening syndrome involving dysfunction of multiple immune cell types.

Objective

This study aimed to comprehensively depict the dynamic trajectory of immune responses throughout the disease course of HBV-related ACLF (HBV-ACLF).

Design

Single-cell RNA sequencing and single-cell proteomics were performed on the peripheral blood mononuclear cells of 45 samples from 17 patients who were hospitalised (progressive/stable/recovering course of HBV-ACLF, 6/5/6) and 15 control subjects (liver cirrhosis, chronic hepatitis B and healthy controls, 5/5/5). Functional and mechanistic experiments were validated in vivo and in vitro.

Results

Single-cell multiomics analysis revealed specific changes in the peripheral

SUBMITTER: Liang X 

PROVIDER: S-EPMC12916473 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature