NRAV promotes HCC stemness via the m6A-regulated let-7c-5p/LIN28B axis.

ABSTRACT: Long non-coding RNAs (lncRNAs) plays critical roles in hepatocellular carcinoma (HCC), but their post-transcriptional regulation via N6-methyladenosine (m6A) remains poorly understood. Here, we identify NRAV as an m6A-modified lncRNA that promotes HCC stemness through a defined molecular axis. Integrating bioinformatics analysis of TCGA data with in vitro and in vivo validation, we demonstrated that NRAV is significantly overexpressed in HCC and associated with poor prognosis. Mechanistically, NRAV acts as a competing endogenous RNA (ceRNA), sequestering hsa-let-7c-5p and preventing it from downregulating LIN28B, a key stemness factor. This leads to increased LIN28B protein levels and enhanced expression of cancer stem cell markers. MeRIP-qPCR and site-directed mutagenesis experiments conf

SUBMITTER: Chen Y 

PROVIDER: S-EPMC12916485 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature