Evaluation of DMSO-free cryopreservation reagent XT-Thrive for establishment of mesenchymal stem cell bank platform.

ABSTRACT:

Introduction

Effective cryopreservation is essential for the clinical application and large-scale banking of mesenchymal stem cells (MSCs). This study compares the performance of a novel DMSO-free cryoprotectant, XT-Thrive®, with a conventional DMSO-based solution, CryoStor® CS10, in preserving both commercial and donor-derived bone marrow MSCs (BM-MSCs). Evaluations focused on viability, recovery, proliferation, and functional characteristics across master cell bank (MCB), working cell bank (WCB), and final product (FP) stages.

Methods

In Part 1, commercial BM-MSCs were cryopreserved in XT-Thrive or CS10 and evaluated for pre-freeze viability, post-thaw survival (up to 6 h), and recovery in 2D and 3D cultures. In Part 2, donor-derived BM-MSCs were cryopreserved at passages

SUBMITTER: Lam AT 

PROVIDER: S-EPMC12916550 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature