Recombinant ROP8 antigen: diagnostics, immunogenicity, and therapeutic targeting in toxoplasmosis.

ABSTRACT:

Introduction

Toxoplasma gondii invasion relies on the battery of specialized proteins, such as ROP8, which plays a key role in formation of parasitophorous vacuoles within host cells. The aim of the work was to evaluate diagnostic, immunoprophylactic and therapeutic potential of recombinant ROP8 (rROP8) protein as despite many years of research this parasitosis still is considered a significant medical and economic problem and the main areas requiring improvement are the diagnostics of the infection, vaccine development especially for humans and safe, specific chemotherapy.

Methods

Recombinant ROP8 protein (rROP8) of Toxoplasma gondii was cloned, expressed in E. coli Rosetta(DE3)pLysS, and purified by nickel-affinity chromatography. Its diagnostic potent

SUBMITTER: Chyb M 

PROVIDER: S-EPMC12916717 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature