Spatial profiling of longitudinal glioblastoma reveals consistent changes in cellular architecture, post-treatment.

ABSTRACT:

Background

Glioblastoma (GBM), the most aggressive adult brain cancer, comprises a complex tumor microenvironment (TME) with diverse cellular interactions that drive progression and pathobiology. The aim of this study was to understand how these spatial patterns and interactions evolve with treatment.

Methods

To explore these relationships, we employed imaging mass cytometry to measure the expression of 34 protein markers, enabling the identification of GBM-specific cell types and their interactions at the single-cell protein level in paired primary (pre-treatment) and recurrent (post-treatment) GBM samples from five patients.

Results

We find a significant post-treatment increase in normal brain cells alongside a reduction in vascular cells. Moreover, despite minimal

SUBMITTER: Ajaib S 

PROVIDER: S-EPMC12916744 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature