Proteomic signatures of sperm and extracellular vesicles associated with sperm freezability in Holstein bulls.

ABSTRACT: Semen cryopreservation and artificial insemination have crucial and beneficial effects on cattle breeding. The freezability of sperm, as primarily reflected by post-thaw sperm motility (PTM), is essential for evaluating semen quality. Some studies have shown notable differences in sperm freezability among various bulls. Here, we compared protein profiles of sperm cells and seminal plasma extracellular vesicles (SPEVs) in the high sperm freezability group and the low sperm freezability group of Holstein bulls to identify the important proteins and their mechanisms that affect sperm freezability. As a result, 432 and 394 differentially expressed proteins (DEPs) were identified in sperm and SPEVs between high and low freezability groups. The results of weighted correlation network analysis (W

SUBMITTER: Cao J 

PROVIDER: S-EPMC12916939 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature