Orbitrap Astral-based proteome and phosphoproteome analysis identifies candidate proteins associated with the phosphatidate phosphatase MoPah1 in Magnaporthe oryzae.

ABSTRACT:

Background

Previous studies have shown that the phosphatidate phosphatase MoPah1 plays a critical role in Magnaporthe oryzae development and pathogenicity. The gene deletion mutant exhibits reduced virulence on rice leaves; however, the MoPah1 regulatory network remains unclear.

Result

In this study, we applied a quantitative data-independent acquisition approach using the Orbitrap Astral instrument to analyze the gene deletion mutant. We identified 6,799 proteins and 15,682 phosphorylation sites in M. oryzae, with differentially abundant proteins primarily enriched in metabolic and autophagy pathways. Integrating phosphoproteomic and proteomic analyses, we found 72 overlapping proteins. Additionally, we employed glutathione S-transferase pull-down and yeast two-hybrid assay

SUBMITTER: Zhao J 

PROVIDER: S-EPMC12917106 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature