WTAP-Mediated Glutaminase Splicing Bias Suppresses Ferroptosis in Hepatocellular Carcinoma.

ABSTRACT: Background: Hepatocellular carcinoma (HCC), a highly aggressive malignancy with poor prognosis, is characterized by hyperactivation of the epidermal growth factor receptor (EGFR) signaling pathway. Glutaminase (GLS) is commonly overexpressed in numerous malignant tumors and acts as an oncogene to support cell growth and tumor progression, making it a target for cancer treatment. This study aimed to elucidate the underlying mechanisms of EGFR activation in driving glutaminolysis reprogramming and conferring ferroptosis resistance in HCC. Methods: Untargeted metabolomics, stable isotope-assisted metabolomic analysis, and RNA sequencing analysis were utilized to elucidate the mechanisms underlying glutaminolysis reprogramming upon EGFR activation. Immunoprecipitation, RNA pulldo

SUBMITTER: Zhu C 

PROVIDER: S-EPMC12917114 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature