Engineering energy-efficient Saccharomyces cerevisiae for methanol and CO₂ assimilation.

ABSTRACT: Methanol is a promising one-carbon (C1) feedstock for microbial bioconversion; however, engineered Saccharomyces cerevisiae often faces energetic constrains during its assimilation. Here, we develop SC-AOX₂₅, an energy-efficient methylotrophic S. cerevisiae, through engineering of heterologous methanol-formaldehyde-formate (MFF) oxidation pathways coupled with adaptive laboratory evolution. SC-AOX₂₅ efficiently generates adenosine triphosphate (ATP) and nicotinamide adenine dinucleotide (NADH) during methanol metabolism while co-assimilating methanol-derived intermediates (formaldehyde, formate, and CO₂) via native glyoxylate-serine cycle, pentose phosphate pathway, and reductive glycine pathway. Key energy modules - Fdh1_sc, Adh2_m, Aox_m

SUBMITTER: Zhong W 

PROVIDER: S-EPMC12917282 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature