The development of a methodology to measure primary ciliary length in neural progenitor cells with a target molecule knocked down.

ABSTRACT: The primary cilium is an immotile cellular antenna that extends from the basal body and protrudes from the cell surface during the G₀/G₁ phases. The cilium is resorbed when it receives growth factor stimuli, and the ciliary resorption triggers the cell cycle re-entry into the G₁/S phases. The dysregulation of ciliary dynamics during embryonic development can lead to various hereditary organ dysplasias, including microcephaly. Neural progenitor cells display primary cilia on their apical surfaces during the embryonic stage. Ciliary resorption in the cells is responsible for cell proliferation and corticogenesis. However, the molecular mechanisms underlying ciliary resorption in the neural progenitor cells in vivo are poorly understood. Mapping cilia t

SUBMITTER: Saito M 

PROVIDER: S-EPMC12917381 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature