Bridging light and sound: A spironaphtopyran-rhodamine dyad with high-contrast photoswitching between fluorescence and photoacoustic signal.

ABSTRACT: Fluorescence and photoacoustic imaging are complementary modalities that provide distinct advantages for biological imaging: fluorescence microscopy offers high sensitivity and resolution, while photoacoustic imaging enables deeper penetration in complex tissue. Leveraging the strengths of both modalities through optically switchable contrast agents can offer enhanced imaging contrast and facilitate dual-modality imaging. Here, we report a photoswitchable probe capable of toggling between high fluorescence and high photoacoustic signal upon illumination, exploiting Förster Resonance Energy Transfer (FRET). We engineer novel spironaphtopyran photoswitches which undergo reversible photoisomerization between absorbing and non-absorbing states. Their photoswitching properties were systematical

SUBMITTER: Kaydanov N 

PROVIDER: S-EPMC12917386 | biostudies-literature | 2026 Apr

REPOSITORIES: biostudies-literature