Molecular detection of filarioid nematodes (Nematoda: Onchocercidae) in wild mammals from different Brazilian biomes.

ABSTRACT: Parasitic nematodes within Onchocercidae are a diverse group transmitted by hematophagous arthropods. This study investigated the molecular occurrence of filarioid nematodes in 93 wild mammals from the Amazon, Cerrado and Pantanal biomes in Brazil, based on the analysis of the mitochondrial genes 12S ribosomal DNA gene (12S rDNA) and cytochrome c oxidase subunit I gene (COI). Conventional polymerase chain reaction (cPCR) targeting the 12S rDNA gene yielded positive results in 14·44% (13/93) of the samples, including 9·86% of jaguars (7/71), 50% of pumas (1/2), 12·5% of giant anteaters (1/8), 50% of ocelots (1/2) and 60% of crab-eating foxes (3/5). Among the 12S-positive samples, 46% (6/13) also tested positive for the COI gene; however, only 1 sequence was suitable for further analysis. Ph

SUBMITTER: Santana MS 

PROVIDER: S-EPMC12917412 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature