Root tip excision-induced exodermis lignification impacts lateral root emergence in Brachypodium distachyon.

ABSTRACT: The mechanisms controlling lateral root emergence in monocots, particularly the role of the exodermis, are poorly understood. We investigated how natural variation in the Brachypodium distachyon stress response shapes root system architecture by modulating cell wall dynamics. We used root tip excision to synchronize lateral root development across natural accessions. The resulting phenotypes were analysed using comparative transcriptomics, biochemical lignin quantification, confocal Raman spectroscopy, and chemical inhibition of lignin biosynthesis. Two distinct root system architectures, 'pine tree' and 'fishbone', were identified. The 'fishbone' phenotype results from an altered lateral emergence caused by a differential lignification intensity of the exodermis. This phenotype was accomp

SUBMITTER: Bellande K 

PROVIDER: S-EPMC12917469 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature