The Oxytocin Receptor in Spermatozoa May Originate From Both Spermatogenesis and Epididymal Maturation, and Regulates Capacitation.

ABSTRACT:

Background

The oxytocin receptor (OR) is a G-protein-coupled receptor recently identified in human spermatozoa, whose origin and role in sperm physiology remain unknown.

Objectives

In this study, using the pig as a model, we examine the presence of the OR in ejaculated spermatozoa through immunofluorescence and immunoblotting, and investigate the receptor's origin in the male gamete via immunohistochemistry in testicular and epididymal tissues. Additionally, we assess the involvement of the OR in in vitro capacitation and the acrosome reaction by utilizing physiological concentrations of agonists (oxytocin and carbetocin) and an antagonist (L-371,257).

Results

The results indicate that, in addition to the expected presence in ejaculated spermatozoa, the OR is expresse

SUBMITTER: Martinez-Hernandez J 

PROVIDER: S-EPMC12917578 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature