SLC25A37 as a novel therapeutic target for benign prostatic hyperplasia: integrative analyses of single-cell RNA sequencing and genome-wide association studies.

ABSTRACT:

Objectives

To identify causal genes for benign prostatic hyperplasia (BPH) based on single-cell RNA sequencing (scRNA-seq) and genome-wide association studies.

Methods

We explored scRNA-seq datasets to identify the differentially expressed genes (DEGs) in BPH patients vs. healthy controls. Mendelian randomization (MR) was conducted to investigate the causal relationships between the identified DEGs and BPH. Bayesian colocalization and reverse MR were performed to consolidate the MR findings. Potential therapeutic drugs targeting causal genes were explored via molecular docking. The results of bioinformatics analyses were validated through experiments including flow cytometry, quantitative reverse transcription polymerase chain reaction, western blotting, immunohistochemistry

SUBMITTER: Tong Z 

PROVIDER: S-EPMC12917586 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature