Detection of Organelle-Specific Dyes Labeled Extracellular Vesicles with Colocalization-Fluorescence Nanoparticle Tracking Analysis.

ABSTRACT: Most cell types release a diverse array of extracellular vesicles (EVs) that contribute to intercellular communication. In particular, considering the heterogeneity of EVs, methods capable of identifying and measuring individual vesicles are limited. Here, we used fluorescence and colocalization Nanoparticle Tracking Analysis (NTA) to identify the subcellular origin of vesicles and determine their physical characteristics, as well as colocalization ratios of endoplasmic (ER) and Mitochondria (Mito) positive EVs in human choriocarcinoma cells (JAr) and bovine follicular fluids (BFF). The labeling efficiency for ER-labeled JAr EVs purified in SEC was 67.11 ± 25.40%, compared to 96.27 ± 13.72% of BFF EVs. Regarding Mito dye labeling efficiency, SEC-purified BFF EVs (14.21 ± 7.45%) provided lo

SUBMITTER: Midekessa G 

PROVIDER: S-EPMC12917633 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature