Two-Step Algorithmic Selection of Interspecies Sequence-Mismatch-Based Housekeeping Genes for Precise Gene-Expression Assessment in PBMC-Humanized NSG Mice.

ABSTRACT: Accurate normalization of gene expression is crucial in humanized mouse models, in which human cells are engrafted into immunodeficient mice. However, selecting the appropriate housekeeping genes remains a challenge. We aimed to identify and validate robust housekeeping genes for gene-expression analysis for human cells residing in different tissues of human peripheral blood mononuclear cell (hPBMC)-humanized NSG mice. We employed a multifaceted approach combining computational analysis and in vivo validation. The expression of 10 candidate genes (EEF2, EIF2S3, GDI1, MYH9, SF3B1, SPCS3, ACTB, SDHA, TBP, and TFRC) was evaluated for stability in human PBMCs using five algorithms (Geomean, Average SD, NormFinder, GeN

SUBMITTER: Jo M 

PROVIDER: S-EPMC12917718 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature