Biosensor Based on pSi-SU-8-GQD Heterostructures for Enhanced Viral Detection by Dual Signal Amplification Strategy.

ABSTRACT: DNA microarray technology requires optimizing substrate properties and DNA labeling strategies for enhanced detection. This study introduces an improved biosensor platform utilizing nanoporous silicon (pSi) substrates with a glycidyl ether of bisphenol A (SU-8) polymer coating, decorated with graphene quantum dots (GQDs), to amplify fluorescent signals. We compared two methods for obtaining labeled single-stranded DNA: Lambda exonuclease digestion of phosphorylated PCR products and asymmetric PCR (aPCR) with Cy5-labeled nested primers. Our findings demonstrate that pSi biochips with 7 nm pores covered by thicker SU-8 layers (700-750 nm) provide 52% higher signal relative to the standard deviation. The aPCR labeling method demonstrated superior performance, yielding significantly stronger s

SUBMITTER: Popescu MA 

PROVIDER: S-EPMC12917824 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature