Unveiling the early defense response dynamics in grapevines against Plasmopara viticola by single-cell transcriptomics.

ABSTRACT:

Background

The viticulture has long suffered from the downy mildew caused by Plasmopara viticola, a strictly obligate biotrophic oomycete. Numerous studies have been performed to reveal how grapevine defends against Plasmopara viticola, but they mainly investigate the plant defense responses on the whole tissue level, not on the cellular level.

Results

Here we employ single-cell RNA sequencing and spatial RNA sequencing to profile approximately 100,000 individual cells (~ 89,000 from scRNA-seq and ~ 11,000 from spRNA-seq), generating the first single-cell transcriptome atlas of grapevine leaves during Plasmopara viticola infection. This high-resolution atlas reveals the dynamic and distinct defense responses of plant cells at early stages of oomycete infection. Notably, we f

SUBMITTER: Yao X 

PROVIDER: S-EPMC12918531 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature