Elevated miR-409-5p may promote the progression of osteoarthritis by targeting DLST as a potential biomarker function of miR-409-5p in osteoarthritis.

ABSTRACT:

Background

Osteoarthritis (OA) significantly impairs the quality of life of middle-aged and elderly individuals. MicroRNAs (miRNAs) are known to play key regulatory roles in OA progression.

Aims

To investigate the potential role mechanisms of miR-409-5p in OA.

Methods

This study enrolled 93 OA patients and 75 non-OA controls. The expression levels of miR-409-5p, DLST, and OA-related markers were measured using RT-qPCR or Western Blot. The targeting relationship between miR-409-5p and DLST was verified by dual luciferase reporter assays. Cell viability was assessed with the CCK-8 assay, and inflammatory cytokine concentrations were measured via ELISA.

Results

Serum miR-409-5p expression was higher in OA patients than in controls and showed good diagnostic value

SUBMITTER: Zheng J 

PROVIDER: S-EPMC12918639 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature