Nanopore Label-Free Monitoring of B-DNA and Z-DNA Conformational Transition in Real Time.

ABSTRACT: The transition from right-handed B-DNA to left-handed Z-DNA represents one of the most dramatic structural changes in biology and plays a crucial role in gene expression and transcription. Monitoring this transition is essential for understanding fundamental biological processes, elucidating disease mechanisms, and developing more effective therapeutic strategies. Here, we present a label-free approach to distinguish between Z-DNA and B-DNA by detecting changes in the electrical current modulations as they translocate through a nanopore. This method enables real-time monitoring of B-to-Z and Z-to-B transitions and studying reaction kinetics at the single-molecule level. By directly capturing dynamic structural changes in DNA, our nanopore platform offers a powerful way to investigate DNA s

SUBMITTER: Zheng H 

PROVIDER: S-EPMC12918715 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature