Posttranscriptional Modification to Modulate Progenitor Differentiation on Heterotypic Spheroids.

ABSTRACT: Cell aggregates are widely used to study heterotypic cellular interactions during the development of vascularization in vitro. In this study, we examined heterotypic cellular spheroids made of adipose-derived stem cells and CD34⁺/CD31^- endothelial progenitor cells induced by the transfection of miR-148b mimic for de novo induction of osteogenic differentiation and miR-210 mimic for de novo induction of endotheliogenesis, respectively. The effect of the microRNA (miRs) mimic treatment group and induction time on codifferentiation was assessed in spheroids formed of transfected cells over the course of a 4-week culture. Based on gene and protein markers of osteogenic and endotheliogenic differentiation, as well as mineralization assays, our results

SUBMITTER: Celik N 

PROVIDER: S-EPMC12918774 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature