Effect of Passaging on Bovine Chondrocyte Gene Expression and Engineered Cartilage Production.

ABSTRACT: Tissue engineering strategies show great potential for repairing osteochondral defects in osteoarthritic joints; however, these approaches often rely on passaging cells multiple times to obtain enough cells to produce functional tissue. Unfortunately, monolayer expansion culture causes chondrocyte dedifferentiation, which is accompanied by a phenotypical and morphological shift in chondrocyte properties that leads to a reduction in the quality of de novo cartilage produced. Thus, the objective of this study was to evaluate transcriptional variations during in vitro expansion culture and determine how differences in cell phenotype from monolayer expansion alter development of functional engineered cartilage. We used an unbiased approach to explore genome-wide transcriptional d

SUBMITTER: Lindberg ED 

PROVIDER: S-EPMC12918923 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature