Rapid bacteriophage quantification with a particle size analyzer combined with polarization intensity differential scattering (PIDS) detector.

ABSTRACT: The study of bacteriophages (phages) and effects on their microenvironments expanded exponentially within the last decade. While there are multiple described methods for phage quantitation, there is still a need for a rapid, label-free method. To this end, we established a procedure for rapid phage quantitation through novel use of a particle size analyzer with Polarization Intensity Differential (PIDS) technology and eliminated the need for labels or knowledge of bacterial host. We validated the procedure and analysis method, termed PhageFOTO (Fast Optical Tallying of Objects) using several physiologically different phages ranging from ~6 nm capsid width (Inoviridae) to ~90 nm capsid width (Caudoviricetes). PhageFOTO demonstrated 89 ± 4.3%, 98 ± 1.7%, and 94 ± 2.7% accuracy for quantitati

SUBMITTER: Carroll-Portillo A 

PROVIDER: S-EPMC12919812 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature