Uncovering the hidden yeast diversity in fermented coffee: Insights from a shotgun metagenomic approach.

ABSTRACT: Yeasts play a pivotal role in coffee fermentation, shaping microbial succession and contributing to the development of final flavor profiles. Despite their importance, yeast taxonomy in this context remains poorly resolved. Traditional classification methods often result in misidentifications due to the limited resolution of classical microbiological techniques and the rapidly evolving taxonomic framework driven by advances in phylogenomic. Moreover, the diversity of budding yeasts in coffee fermentations remains underexplored using high-resolution approaches such as metagenomics. To address this gap, we applied a shotgun metagenomic strategy and reconstructed metagenome-assembled genomes (MAGs) from multiple coffee fermentation samples and, using a robust phylogenomic framework based on 8

SUBMITTER: Bedoya-Urrego K 

PROVIDER: S-EPMC12919822 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature