Insights into the olaparib-mediated cell death mechanisms in canine hematological malignancies: a different fate for CLBL-1 and GL-1 cell lines.

ABSTRACT:

Introduction

Olaparib (OLA) is a poly ADP-ribose polymerase inhibitor (PARPi) indicated for solid cancers harboring BRCA1/2 mutations. Recent evidences suggest that sensitivity to PARPis may also be influenced by other factors that impair the DNA repair mechanisms. Since various hematological malignancies exhibit these types of defects, this study aims to investigate further the mechanism of action of OLA in CLBL-1 and GL-1 canine cell lines, which showed different sensitivities to this PARPi.

Methods

CLBL-1 and GL-1 cell lines were exposed to OLA (12.5, 25, and 50 μM) for 24 and 48 h and were subjected to preliminary cell death evaluations by flow cytometry. Then, both immunoblotting for the assessment of Bcl-2 and Bcl-XL, and RNA-seq were carried out after 24 h of e

SUBMITTER: Mucignat G 

PROVIDER: S-EPMC12920183 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature