Proinflammatory cytokines modify Ca²⁺ signaling and induce an apoptotic lipidome in murine small intestinal organoids.

ABSTRACT:

Background

Inflammatory bowel diseases (IBD) are associated with elevated levels of proinflammatory cytokines exerting a detrimental effect on the intestinal epithelium. Since contradictory studies regarding time-dependent actions on epithelial secretion exist, intestinal organoids were treated with a mix of proinflammatory cytokines.

Methods

Long-term (1-5 days) effects of a cytokine mix consisting of tumor necrosis factor α, interleukin-1β and interferon-γ on murine small intestinal epithelial organoids were investigated using live cell imaging, immunofluorescent staining, qPCR, scanning microprobe matrix-assisted laser desorption/ionization mass spectrometry imaging (AP-SMALDI MSI), and liquid-chromatography tandem mass spectrometry (UHPLC MS/MS).

Results

Treatment

SUBMITTER: Pauer SM 

PROVIDER: S-EPMC12920202 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature