Incision of the pre-Descemet layer and Descemet membrane affects outflow facility: <i>ex-vivo</i> studies on a human eye perfusion model.

ABSTRACT:

Purpose

The collagen and elastin fibres of the pre-Descemet layer or Dua layer (PDL) fan out to become the beams of the trabecular meshwork (TM), indicating similarities in the structural elements in the PDL and TM. This study evaluated the effects of disruption of the PDL and Descemet membrane (DM) on the facility of aqueous outflow from the anterior chamber, using human eyes and an anterior segment perfusion model (ASPM).

Methods

The ASPM was established using human donor eyes. The PDL and DM in the experimental eyes were half incised circumferentially along an 8 mm diameter circle for 180°, and the other half was left non-incised.

Results

The experimental eyes in the ASPM model exhibited a decrease in the outflow rate, indicating an increased outflow resistance. In

SUBMITTER: Sahay P 

PROVIDER: S-EPMC12920517 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature