<i>In silico</i> design of a multiepitope subunit vaccine targeting <i>Salmonella enterica</i> serovar Infantis: an immunoinformatics and reverse vaccinology approach.

ABSTRACT: Salmonella enterica serovar Infantis is an emerging zoonotic pathogen increasingly linked to poultry and multidrug-resistant human infections. Existing vaccines lack serovar-specific efficacy, underscoring the need for targeted immunization strategies. In this study, we employed an immunoinformatics and reverse vaccinology pipeline to design a multiepitope subunit vaccine (MEV) against S. Infantis. A subtractive proteomics analysis of 692 poultry-derived genomes identified CsgA as a highly conserved, immunogenic, and non-host homologous antigen. Selected cytotoxic T lymphocyte (CTL), helper T lymphocyte (HTL), and B-cell epitopes were filtered for antigenicity, immunogenicity, allergenicity, and sequence conservation. The epitopes were assembled with appropriate linkers and a

SUBMITTER: Chundru D 

PROVIDER: S-EPMC12920593 | biostudies-literature | 2026

REPOSITORIES: biostudies-literature