Paired Duodenal and Salivary Microbiome Analysis in Pancreatic Cancer Without Duct Obstruction.

ABSTRACT:

Background

Bacterial migration from the oral cavity to the upper gastrointestinal tract has been proposed as a contributor to pancreatic ductal adenocarcinoma (PDAC) onset and prognosis. Whether PDAC is associated with alterations of the oral-duodenal microbiome continuum remains unclear.

Methods

In this prospective study, we profiled matched saliva and duodenal brushings from 24 treatment-naïve PDAC patients without ducts obstruction and 24 age- and sex-matched healthy controls (HC). Microbial composition was assessed by 16S rRNA gene sequencing. α-Diversity was evaluated using Faith's phylogenetic diversity (PD), observed ASVs, and Pielou's evenness; β-diversity using UniFrac, Bray-Curtis, and distance-based redundancy analysis (db-RDA). Associations with overall survival

SUBMITTER: Archibugi L 

PROVIDER: S-EPMC12920678 | biostudies-literature | 2026 Mar

REPOSITORIES: biostudies-literature