Determination of hydrocortisone and cortisone in artificial saliva by spray assisted fine droplet formation liquid phase microextraction coupled to LC-MS/MS.

ABSTRACT: Hydrocortisone and cortisone are clinically informative steroids whose joint measurement at low concentrations is essential for ratio-reliable interpretation. A spraying-assisted fine droplet formation liquid-phase microextraction (SA-FDF-LPME) was combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) for simultaneous quantitation in saliva-type matrices as part of a method-development study. Method parameters were optimized to secure chromatographic resolution, stable ion ratios, and high sensitivity. Under direct injection, LOD/LOQ values were 1.05/3.50 µg kg^-1 for cortisone and 0.80/2.68 µg kg^-1 for hydrocortisone, with dynamic ranges of 3.65-150.77 and 2.59-268.45 µg kg^-1, respectively. With SA-FDF-LPME coupled to LC-MS/MS, LOD/LOQ wer

SUBMITTER: Gursoy S 

PROVIDER: S-EPMC12921225 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature