Label-free blood cell separation for space health monitoring using a portable blast cell biochip.

ABSTRACT: We demonstrate a novel biomedical application of a commercial spiral microfluidic chip (Fluidic 382), originally developed for particle sorting, by repurposing it for label-free, size-based isolation of pathological blood cells, including leukemic blasts from acute myeloid leukemia (AML) samples. For the first time, we establish and validate a streamlined protocol for cell separation using Dean-driven hydrodynamic forces in a chip not originally designed for blood analysis. Using a 9-turn, 6-outlet spiral channel configuration, we achieved high-efficiency sorting of red and white blood cells from healthy donors and selectively enriched pathological blasts from AML patient blood. The device's performance was validated through flow cytometry and numerical simulations, demonstrating strong ag

SUBMITTER: Mugnano M 

PROVIDER: S-EPMC12921280 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature