Transcriptomic and Metabolic Insight Into Flavonoid Biosynthesis Underlying Black and Yellow Seed Coat Color Variation in Soybean (<i>Glycine max</i>).

ABSTRACT: Soybean (Glycine max) seed coat color variation is determined by the accumulation of flavonoid-derived pigments, although the molecular mechanisms underlying this trait remain poorly understood. This study integrated RNA sequencing (RNA-Seq) and high-performance liquid chromatography (HPLC)-based metabolite measurements to investigate black and yellow seed coat soybean lines derived from the same genetic background. Metabolite analysis revealed significantly higher total phenolic content (TPC), total flavonoid content (TFC), total anthocyanin content (TAC), total proanthocyanidin content (TPAC), and antioxidant activity (DPPH, ABTS) in black seed coats, whereas yellow seed coats exhibited elevated total isoflavone content (TIC). RNA-seq at 110 days after sowing (DAS) identified diff

SUBMITTER: Moon K 

PROVIDER: S-EPMC12921420 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature