High-Quality Genome Assembly and Annotation of Ceratonia siliqua Provide Insights Into the Secondary Loss of Symbiotic Nitrogen Fixation.

ABSTRACT: The carob tree (Ceratonia siliqua L.), an evergreen legume native to West Asia and long cultivated throughout the Mediterranean basin, is valued for its drought tolerance, nutritious pods, and ecological value. Despite its economic and environmental importance, genomic resources for this species have been limited. Here, we present a high-quality, chromosome-scale genome assembly of C. siliqua, generated using PacBio HiFi long-read and Hi-C sequencing technologies. The final assembly spans 501.39 Mb, organized into 12 pseudomolecules, with a scaffold N50 of 39.58 Mb. Genome annotation identified 30,295 protein-coding gene models, with 99.5% completeness according to conserved single-copy orthologs. Repetitive elements account for 52.2% of the genome, primarily long terminal repeat (LTR) ret

SUBMITTER: Bibi AC 

PROVIDER: S-EPMC12921467 | biostudies-literature | 2026 Jan-Feb

REPOSITORIES: biostudies-literature