MSumireF a Monomeric Violet Fluorescent Protein.

ABSTRACT: The development of genetically encoded labels for multicolor experiments requires a diverse palette of fluorescent proteins that are well-behaved. Here, we report mSumireF, a monomeric variant of the violet fluorescent protein Sumire. On its own, the canonical monomerizing valine-to-lysine mutation at residue 206 causes a significant loss of brightness for the mSumire variant. We found that brightness is recovered upon the reversion of mSumire's tyrosine at position 165 back to phenylalanine as in its superfolder GFP grandparent. Importantly, this mSumireF variant exhibits significantly less oligomerization tendency in the organized smooth endoplasmic reticulum (OSER) assay in mammalian cells and in protein solution assays. Furthermore, we demonstrate that an mSumireF donor can be effectiv

SUBMITTER: Kress J 

PROVIDER: S-EPMC12921512 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature