Precision mRNA Delivery via Ultrasound-Controlled Release Perfluorocarbon Emulsions: An Innovative Ultrasound Theranostic Strategy with ¹⁹F MRI Feasibility.

ABSTRACT: Messenger RNA (mRNA) therapeutics provide promising opportunities in cardiovascular diseases. However, effective vascular mRNA delivery requires precise delivery, controlled release, and efficient transfection. To address these challenges, the study utilizes phase-change perfluorocarbon nanodroplets (PFC NDs) as a unique theranostic platform, integrating multimodal imaging with ultrasound-triggered mRNA release for enhanced vascular transfection. Lipid-coated PFC NDs are engineered using perfluoro-crown-ether (PFCE), perfluorohexane (PFH), and perfluoropentane (PFP) as core materials. These nanodroplets (200-300 nm) are optimized for mRNA loading and cellular uptake, exhibiting strong ultrasound contrast in tissue-mimicking phantoms and in vivo. Each PFC generated distinct fluorine-19 magn

SUBMITTER: Liu H 

PROVIDER: S-EPMC12921544 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature