A Systematically Enhanced LAMP Chip for Rapid, Sensitive, and Contamination-Free Pathogen Detection.

ABSTRACT: Main techniques for pathogen detection include molecular or nucleic acid-based methods, such as polymerase chain reaction (PCR) and loop-mediated isothermal amplification (LAMP). Among these, chip-based LAMP provides a particularly promising platform for point-of-care diagnostics. To ensure accurate, reliable, and robust pathogen detection, systematic optimization of the LAMP-chip system is essential. Building on our previously developed LAMP-chip platform, we report a systematically advanced version for the rapid and sensitive detection of Phytophthora infestans. By systematically investigating the effects of primer concentrations, immobilization ratios, and reaction conditions, we identified an optimal configuration involving immobilized forward inner primers (FIP) on the sensor s

SUBMITTER: Uchayash S 

PROVIDER: S-EPMC12921607 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature