SERS-Based Immunoassay on Ag/ZnO Nanorod Substrates for Detection of CA125 Antigen.

ABSTRACT: Several reports have been published on the detection of the carbohydrate antigen 125 (CA125) cancer biomarker, where the immunoassay is completed with a molecule tag that is detected via surface-enhanced Raman scattering (SERS); however, it is still challenging to detect protein biomarkers without a Raman reporter. In this study, a SERS substrate based on zinc oxide nanorods (ZnO NRs) decorated with silver nanoparticles was fabricated, functionalized, and bioconjugated to detect CA125. Functionalization was performed by using an MPA self-assembled monolayer, which was subsequently surface-activated with an EDC/NHS solution. This process was optimized by using Raman measurements to determine the surface protonation of the substrate. The effect of the concentration and incubation time of the

SUBMITTER: Zamora-Peredo L 

PROVIDER: S-EPMC12921610 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature