Benchmarking Lysosome Enrichment Methods: A Guide for Research and Clinical Translation.

ABSTRACT: Lysosomes, essential organelles involved in diverse cellular processes, are increasingly recognized as central players in the pathogenesis of numerous diseases. Due to their low abundance in whole-cell extracts, enrichment strategies are required for downstream analyses such as proteomics. Despite the availability of various lysosome isolation methods, including density gradient-based separation, filter-based approaches, magnetic bead-based isolation, and subcellular fractionation, a systematic, multimodal comparison of their performance is lacking. Here, four widely used lysosome enrichment techniques are benchmarked using the THP-1 monocytic cell line as a model. Each method has been evaluated for yield, purity, membrane integrity, reproducibility, scalability, and cross-contamination, e

SUBMITTER: de Jager AL 

PROVIDER: S-EPMC12921670 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature