Quantum Spin Detection in Microfiltration Immunoassays for Ultrasensitive and High-Throughput Diagnostics.

ABSTRACT: This study demonstrates the transformative potential of quantum technologies for healthcare diagnostics by developing a new analytical method, the quantum-enabled microfiltration immunoassay (QEMFIA). QEMFIA integrates the strengths of dot blot and enzyme-linked immunosorbent assays, enabling rapid, sensitive, and quantitative detection of clinically relevant antigens using nanoscale quantum sensors in a high-throughput format. The assay leverages fluorescent nanodiamonds (FNDs) with nitrogen-vacancy centers as reporters, combined with magnetically modulated fluorescence (MMF) for background-free detection of optically addressable spin defects. Additionally, to achieve high-throughput operation, the assays are performed on a 24-well microfiltration manifold, with target antigens captured b

SUBMITTER: Le TN 

PROVIDER: S-EPMC12921671 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature