Promiscuous Transferases Malonylate Furaneol Glucoside in <i>Fragaria</i> × <i>ananassa</i>.

ABSTRACT: Acylation is essential in plant metabolism, protecting metabolites from enzymatic degradation, aiding xenobiotic detoxification, and regulating cellular uptake. It also enhances the stability, solubility, and bioactivity of natural products, making it valuable for drug discovery. Since HDMF (4-hydroxy-2,5-dimethyl-3(2H)-furanone; Furaneol) 6'-O-malonyl glucoside was detected in strawberries, we hypothesized that strawberry malonyltransferases (FaMATs) acylate HDMF glucoside. Genome analysis of Fragaria × ananassa and biochemical assays identified FaMAT1C, FaMAT1S, and FaMAT4C₁/S₁ as enzymes catalyzing its malonylation, producing three isomers─likely due to keto-enol tautomerism. A screening revealed the broad substrate tolerance of FaMATs,

SUBMITTER: Wulanjati MP 

PROVIDER: S-EPMC12921856 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature