In Vitro 3D Model of Human Endometrial Stromal and Trophoblast Cells: Techniques for an Optimized Formation and Cryopreservation of Spheroids.

ABSTRACT: Three-dimensional (3D) cell culture models provide physiologically relevant systems that mimic the native endometrial environment better than 2D models and offer reliable platforms to study embryo implantation and maternal-embryo interactions. One widely used 3D culture model is the generation of spheroids. However, standardized and reproducible methods for generating uniform spheroids from trophoblast and endometrial stromal cells are limited. In this study, we established and validated a robust protocol for spheroid formation using human trophoblast (HTR8/SVneo, JEG3) and endometrial stromal (St-T1b, tHESC) cell lines. The protocol was further extended to generate spheroids from decidualized tHESC, representing a novel approach that closely reflects the receptive endometrial environment.

SUBMITTER: Muthuraj K 

PROVIDER: S-EPMC12922085 | biostudies-literature | 2026 Feb

REPOSITORIES: biostudies-literature