Hypercholesterolemia Impairs the Expression of Angiogenic MicroRNAs in Extracellular Vesicles Within Ischemic Skeletal Muscles.

ABSTRACT: Background/Objectives: In severe peripheral artery disease (PAD) with limb ischemia, hypercholesterolemia (HC) is associated with impaired neovascularization. Extracellular vesicles (EVs) are present within ischemic muscles, and they contain microRNAs (miRs) involved in several biological functions, including angiogenesis and neovascularization. Methods: We used a mouse model of PAD and compared the response to hindlimb ischemia in hypercholesterolemic ApoE^-/- vs. normocholesterolemic mice. Next-generation sequencing (NGS) was used to perform full miR expression profiling in ischemic skeletal muscles and in EVs of varying sizes-large EVs (lEVs) and small EVs (sEVs)-within these muscles. Results: We identified several miRs with potential pro-angiogenic

SUBMITTER: Raguema N 

PROVIDER: S-EPMC12922120 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature