Phytochemical study of <i>Homalanthus giganteus</i>: isolation, antiproliferative activity, and computational mechanistic insights of tigliane diterpenes against colorectal cancer.

ABSTRACT:

Context

Colorectal cancer is the third most common and second deadliest cancer worldwide. Late diagnosis, poor outcomes in metastatic cases, and multidrug resistance emphasize the need for new therapies.

Objective

This study aimed to isolate bioactive compounds from Homalanthus giganteus Zoll. ex Miq. (Euphorbiaceae), evaluate their antiproliferative effects on colorectal cancer cells, and identify mechanisms through network pharmacology and molecular docking.

Materials and methods

Compounds were isolated by chromatographic separations, and the structures were elucidated by NMR and HRESIMS. The antiproliferative activity was measured by MTT assay. DFT calculations were performed in ORCA 6.1.0. Network pharmacology was used to identify targets and pathways, mole

SUBMITTER: Wirasisya DG 

PROVIDER: S-EPMC12922413 | biostudies-literature | 2026 Dec

REPOSITORIES: biostudies-literature